Plasmid_Backbone
Part:BBa_K1480018:Design
Designed by: Roberto Alejandro Valderrama Alfaro Group: iGEM14_UANL_Mty-Mexico (2014-10-17)
pSB3C5 + AF and BF TALEN (K1480001) Target sites
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2812
Illegal XbaI site found at 2827
Illegal SpeI site found at 1
Illegal PstI site found at 15 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2812
Illegal NheI site found at 1450
Illegal SpeI site found at 1
Illegal PstI site found at 15
Illegal NotI site found at 8
Illegal NotI site found at 2818 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2812 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2812
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2812
Plasmid lacks a suffix.
Illegal XbaI site found at 2827
Illegal SpeI site found at 1
Illegal PstI site found at 15
Illegal AgeI site found at 1041
Illegal AgeI site found at 1364
Illegal AgeI site found at 3613
Illegal AgeI site found at 3725 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 288
Design Notes
Modifications where inserted by PCR on both directions due to TALEN specificity.
Source
Derived from pSB3C5 plasmid